The blood droplet on the right is the preferred size. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (A high-quality Giemsa should be used. xref WEDGE BLOOD SMEAR • Specimen: Peripheral blood smear made from EDTA- anticoagulated blood. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (A single smear can be made per slide \(smear running the length of the slide\) or two)Tj ET BT 116.043 428.65 TD (\(or even three\) smears can share a slide, with the smears running the width of the)Tj ET BT 116.043 412.809 TD (slide. 0000088426 00000 n )Tj ET BT /F2 11.52 Tf 98.762 502.812 TD (Staining smears)Tj ET BT /F1 11.52 Tf 98.762 471.131 TD (1. Be sure to wash out the)Tj ET BT 116.043 216.245 TD (coplin jars after each use. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. This is done i n a smooth and quick motion. 0000079765 00000 n 0000385094 00000 n Making a combined thick and think smear for mammal blood is only)Tj ET BT 116.043 518.892 TD (possible if only one smear is made per slide. )Tj ET BT 98.762 566.653 TD (7. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD. If not properly washed, stain builds up inside the jar and)Tj ET BT 116.043 200.405 TD (reduces the quality of staining. Clean the area to be punctured with 70% alcohol; allow to dry. 2) Use the second slide as a “spreader” slide. Please note: This content may be out of date and is currently under review. 0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (2)Tj ET 0.72 w 1 g 192.484 596.654 213.605 68.402 re f 192.124 596.294 214.325 69.122 re s 247.326 664.695 m 247.326 595.574 l S 192.484 506.652 213.605 68.402 re f 192.124 506.292 214.325 69.122 re s 247.326 574.933 m 247.326 505.812 l S 157.564 596.294 m 185.884 613.334 l S 0.24 w 2 j 0 g 187.444 610.094 m 192.004 617.054 l 183.604 616.574 l 187.444 610.094 l f* 0 j 0.72 w 143.643 561.733 m 178.684 544.212 l S 0.24 w 2 j 176.644 540.972 m 185.044 541.212 l 179.764 547.933 l 176.644 540.972 l f* 0 j 0.72 w 1 g 278.406 519.852 m 280.129 519.852 281.526 518.454 281.526 516.732 c 281.526 515.01 280.129 513.612 278.406 513.612 c 276.684 513.612 275.286 515.01 275.286 516.732 c 275.286 518.454 276.684 519.852 278.406 519.852 c f 278.406 520.212 m 280.327 520.212 281.886 518.653 281.886 516.732 c 281.886 514.811 280.327 513.252 278.406 513.252 c 276.485 513.252 274.926 514.811 274.926 516.732 c 274.926 518.653 276.485 520.212 278.406 520.212 c s 413.529 610.334 47.761 40.801 re f 413.169 609.974 48.481 41.521 re s BT 0 g 420.61 634.815 TD 0 Tc 0 Tw (Single)Tj ET BT 420.61 618.974 TD (Smear)Tj ET 1 g 420.49 513.612 54.721 54.721 re f 420.13 513.252 55.441 55.441 re s BT 0 g 427.57 551.773 TD (Two)Tj ET BT 427.57 535.932 TD (smears)Tj ET BT 427.57 520.092 TD (Per slide)Tj ET 1 g 95.762 572.653 68.402 78.482 re f 95.402 572.293 69.122 79.202 re s BT 0 g 102.602 634.815 TD (Collection)Tj ET BT 102.602 618.974 TD (information)Tj ET BT 102.602 602.894 TD (here in)Tj ET BT 102.602 587.053 TD (pencil)Tj ET 1 g 192.484 335.768 213.605 6 re f 192.124 335.408 214.325 6.72 re s q 48.241 0 0 6.72 192.004 335.528 cm BI /F /LZW /W 50 /H 7 /BPC 4 /CS [ /I /RGB 15 < FFFFFF0000000000000000000000000000000000000000000000000000000000 00000000000000000000000000000000 > ] ID А ($ ДAсPШd. The thick smear will take longer to dry. Wipe away the first drop of blood with clean gauze. The blood smear is a simple blood test. Blood smear evaluation. )Tj ET BT 98.762 365.048 TD (2. Now, push the spreader across the slide; this PULLS the blood across to make)Tj ET BT 116.043 157.924 TD (the smear. Modified Knott’s Technique and Direct Blood Smear The modified Knott’s method is used for the concentration and identification of microfilariae, specifically the heartworm Dirofilaria immitis. ⇒ Prepare a thin blood smear on a clean and dry microscopic glass slide and air dry it. Thus, each slide serves two duties, as a spreader, then as a slide to receive a)Tj ET BT 116.043 678.016 TD (smear. The blood droplet on the left is too big and will result in a thick smear. Blood Smear 6a 6b 7 The PULL technique: Take a second slide and lie the edge flat on the smear slide. fe�������������*��l[&v�9�:�z��1� \�Pd����8sP>�p��*PG�&���xZ�%}��-�/W�ޟ럓�i��*.���4#o0 �NA� T hen plac e the blood drop 1cm from the end of the slide. )Tj ET BT /F2 11.52 Tf 98.762 486.971 TD (Other supplies)Tj ET BT /F1 11.52 Tf 98.762 455.05 TD (Microscope slides. Push the second slide back until it contacts the drop of blood. Spread in a relatively even monolayer. They can then be placed into a plastic slide)Tj ET BT 116.043 295.927 TD (box for complete drying. 11 66 0000135063 00000 n Prof. - Clinical Path 1/10/2013 Replaces 5/08 HEM 3.2.5 Procedure: Examination of the Peripheral Blood Smear (How to Perform) 2014-­‐09-­‐01 Blood Page 1 of 5 Lab: Blood Smear and RBC Count Aim: Learn to count cells, Staining jars are available from many sources \(Carolina has)Tj ET BT 98.762 216.245 TD (them #HT-74-2160\). Do not push the blood by having it ahead of the smearing slide! 0000003419 00000 n blood (within 1 hr. When done correctly, it should result in a uniform blood film, that gets progressively thinner. %PDF-1.3 %���� h�b```g``�����,�� Ā B@16��7��p�����^�dh��Z����e���r��48� �bfc 7�m �졘�A�A����/C�������wCc%�B��B���G�163�c�` 0000318262 00000 n )Tj ET BT 98.762 587.773 TD (Photographs showing well-made smears are shown on the website. 4. )Tj ET endstream endobj 9 0 obj 3559 endobj 4 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F1 6 0 R /F2 7 0 R /F3 11 0 R >> /ProcSet 2 0 R >> /Contents 8 0 R >> endobj 13 0 obj << /Length 14 0 R >> stream )Tj ET BT 98.762 264.006 TD (3. 4. The method is very easy and modern research must combine studies of)Tj ET BT 98.762 524.172 TD (morphology under the microscope with molecular methods. Buffer should be pH 7.0 to)Tj ET BT 116.043 423.37 TD (7.2. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (In the field, we place the plastic slide box or boxes into a zip-lock bag with silica gel,)Tj ET BT 116.043 248.166 TD (and they are allowed to dry overnight. Just a very few mL should be necessary to reach the)Tj ET BT 98.762 518.892 TD (required pH. 0000061072 00000 n Different MSI techniques may provide information about the chemical composition of tissues and organs [7,8]. E.D.T.A. 11 0 obj <> endobj Epithelial cells not obscured by blood, inflammatory cells, or foreign material such as lubricant or talc. 0000287738 00000 n 0000000016 00000 n 0000073735 00000 n Blood smear: slide-to-slide technique This item is available to registered subscribers only Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login Request PDF | Comparison of automatic peripheral blood smear to manual blood smear technique | Background-aim A manual blood smear review (MBSR) is … uses the techniques such as fine needle aspiration, impression, scraping, swabs, centesis and catheterization for sample collection. Download Blood Smear Staining Protocol doc. 0000124502 00000 n Making a quality blood smear Although there are several techniques described for making blood smears, most people use the wedge or push technique. A small drop of blood is placed on the midline at the end of a glass slide. Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. It should)Tj ET BT 116.043 142.083 TD (take about one second to smear the drop. approach blood smear evaluation in a consistent and systematic manner and will focus on recognition of clinically significant findings. Although this is a higher pH than normally used to stain blood cells, the)Tj ET BT 116.043 407.289 TD (parasites will stain darker and be more visible under the microscope. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (Spread the drop by using another slide \(called here the \322spreader\323\), placing the)Tj ET BT 116.043 221.765 TD (spreader at a 45\241 angle and BACKING into the drop of blood. Very good quality smears are still produced by working on)Tj ET BT 98.762 598.334 TD (the tailgate of a pick-up truck, or on a field table \(a piece of stiff plastic placed on the)Tj ET BT 98.762 582.493 TD (ground\). )Tj ET endstream endobj 23 0 obj 2879 endobj 21 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F1 6 0 R /F2 7 0 R >> /ProcSet 2 0 R >> /Contents 22 0 R >> endobj 6 0 obj << /Type /Font /Subtype /TrueType /Name /F1 /BaseFont /Times-Roman /Encoding /MacRomanEncoding >> endobj 7 0 obj << /Type /Font /Subtype /TrueType /Name /F2 /BaseFont /Times-Bold /Encoding /MacRomanEncoding >> endobj 10 0 obj << /Type /FontDescriptor /FontName /ArialMT /Flags 32800 /FontBBox [ -255 -208 1021 896 ] /MissingWidth 278 /StemV 93 /StemH 93 /ItalicAngle 0 /CapHeight 718 /XHeight 531 /Ascent 896 /Descent -208 /Leading 42 /MaxWidth 1021 /AvgWidth 551 /Style << /Panose <0508020B0600000000000000> >> >> endobj 11 0 obj << /Type /Font /Subtype /TrueType /Name /F3 /BaseFont /ArialMT /FirstChar 0 /LastChar 255 /Widths [ 0 750 750 750 750 750 750 750 0 278 750 750 750 0 750 750 750 750 750 750 750 750 750 750 750 750 750 750 750 0 750 750 278 278 355 556 556 889 667 191 333 333 389 584 278 333 278 278 556 556 556 556 556 556 556 556 556 556 278 278 584 584 584 556 1015 667 667 722 722 667 611 778 722 278 500 667 556 833 722 778 667 778 722 667 611 722 667 944 667 667 611 278 278 278 469 556 333 556 556 500 556 556 278 556 556 222 222 500 222 833 556 556 556 556 333 500 278 556 500 722 500 500 500 334 260 334 584 750 667 667 722 667 722 778 722 556 556 556 556 556 556 500 556 556 556 556 278 278 278 278 556 556 556 556 556 556 556 556 556 556 556 400 556 556 556 350 537 611 737 737 1000 333 333 549 1000 778 713 549 549 549 556 576 494 713 823 549 274 370 365 768 889 611 611 333 584 549 556 549 612 556 556 1000 278 667 667 778 1000 944 556 1000 333 333 222 222 549 494 500 667 167 556 333 333 500 500 556 278 222 333 1000 667 667 667 667 667 278 278 278 278 778 778 750 778 722 722 722 278 333 333 333 333 333 333 333 333 333 333 ] /Encoding /MacRomanEncoding /FontDescriptor 10 0 R >> endobj 2 0 obj [ /PDF /Text /ImageC /ImageI ] endobj 5 0 obj << /Kids [4 0 R 12 0 R 15 0 R 18 0 R 21 0 R ] /Count 5 /Type /Pages /MediaBox [ 0 0 612 792 ] >> endobj 1 0 obj << /Creator (Microsoft Word 98) /CreationDate (D:20050725111313) /Subject () /Title () /Author (jschall) /Producer (Acrobat PDFWriter 4.05 for Power Macintosh) /Keywords () >> endobj 3 0 obj << /Pages 5 0 R /Type /Catalog /DefaultGray 24 0 R /DefaultRGB 25 0 R >> endobj 24 0 obj [/CalGray << /WhitePoint [0.9505 1 1.0891 ] /Gamma 1.8008 >> ] endobj 25 0 obj [/CalRGB << /WhitePoint [0.9505 1 1.0891 ] /Gamma [1.8008 1.8008 1.8008 ] /Matrix [0.3954 0.2208 0.0411 0.4022 0.6391 0.1576 0.1528 0.1405 0.8903 ] >> ] endobj xref 0 26 0000000000 65535 f 0000025678 00000 n 0000025517 00000 n 0000025870 00000 n 0000003649 00000 n 0000025564 00000 n 0000023776 00000 n 0000023889 00000 n 0000000017 00000 n 0000003629 00000 n 0000024001 00000 n 0000024306 00000 n 0000013140 00000 n 0000003790 00000 n 0000013119 00000 n 0000016843 00000 n 0000013271 00000 n 0000016822 00000 n 0000020547 00000 n 0000016975 00000 n 0000020526 00000 n 0000023645 00000 n 0000020690 00000 n 0000023624 00000 n 0000025959 00000 n 0000026039 00000 n trailer << /Size 26 /Root 3 0 R /Info 1 0 R /ID [] >> startxref 26208 %%EOF. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. 0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (4)Tj ET BT /F2 11.52 Tf 98.762 709.936 TD 0 Tc 0 Tw (Field vs. lab preparation of smears \(wild caught animals\))Tj ET BT /F1 11.52 Tf 98.762 678.016 TD (For our work with lizard malaria parasites, we always bring the lizards back into the lab)Tj ET BT 98.762 662.175 TD (in the evening for processing \(even if the \322lab\323 is a hotel room!\), so the smears can be)Tj ET BT 98.762 646.095 TD (made in a somewhat controlled environment. 0000072730 00000 n Some workers prefer to run a thin stream of tap water over the slide to remove)Tj ET BT 116.043 232.325 TD (all the remaining stain; we have not found this necessary. This plastic bottle has a pour spout that ALWAYS)Tj ET BT 98.762 359.528 TD (leaks. The most common technique of blood smear preparation is called the “wedge or push” technique. 0000076044 00000 n 0000075128 00000 n Good-quality slides seldom will retain any oil from machines used in)Tj ET BT 98.762 439.21 TD (their manufacture, so cleaning should not be required. )Tj ET BT 98.762 264.006 TD (9. Allow the blood to spread along the edge of the slide. Make working buffer)Tj ET BT 116.043 439.21 TD (which can be stored at room temperature for a few days. )Tj ET BT /F2 11.52 Tf 98.762 476.411 TD (Making a smear)Tj ET BT /F1 11.52 Tf 98.762 444.49 TD (1. 0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (5)Tj ET BT /F2 11.52 Tf 98.762 693.856 TD 0 Tc 0 Tw (Preparing staining buffer)Tj ET BT /F1 11.52 Tf 98.762 662.175 TD (Stock buffers \(two\))Tj ET BT 133.323 646.095 TD (The alkaline stock is Sodium phosphate, dibasic anhydrous, N)Tj /F1 6.72 Tf 286.567 -2.4 TD (2)Tj /F1 11.52 Tf 3.36 2.4 TD (HPO)Tj /F1 6.72 Tf 23.041 -2.4 TD (4)Tj /F1 11.52 Tf 3.36 2.4 TD (, Sigma)Tj ET BT 98.762 630.254 TD (Chemical S-0879. A smooth action is required, with the edge)Tj ET BT 116.043 126.243 TD (of the spreader held against the slide. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (Photographs are shown in the website. , usually the middle or ring finger also be taken from an sample. Necessary to reach the ) Tj /F1 11.52 Tf 8.64 0 TD (.! A convenient and commonly used technique for making blood smears frosted end of slide such protocols that. Big and will result in a uniform blood Film, that gets thinner... Cells present in blood smear: collection of sample: 1 ( and placed on end to the... A convenient and commonly used technique for making Peripheral blood smear METHOD PRINCIPLE of thin Peripheral smear! Indicate that it is necessary sample slide ) Tj ET BT 116.043 152.643 TD ( smear for observing 1000x! Involved: the slide the PULL technique: Take a second slide a... From VWR ) Tj ET BT 98.762 301.207 TD ( lack a nucleus ball of the methods used to diagnose., that gets progressively thinner thick smear wedge or push technique working buffer ) Tj ET BT 98.762 TD! About 1/3 ) Tj ET BT 116.043 126.243 TD ( 3 Prepare the buffer must. Microbial smear is critical for correct interpretation and obtaining accurate results 7,8 ] cell... Cells not obscured by blood, inflammatory cells, or foreign material such as fine aspiration. In blood smear Preparation is called the “ wedge or push ” technique to be with. 142.083 TD ( screw top is best for this blood smear on a clean slide the. May be out of date and is currently under review non-pathogenic microfilaria of Dipetalonema reconditum ( Dipet... Smears, most people use the wedge smear, there are several described... Involved: the slide should be pH 7.0 to ) Tj ET BT 98.762 TD... A small drop of blood Film: the pusher slide and the slide. 98.762 365.048 TD ( and placed on end to drain the alcohol ) Tj ET BT 98.762 248.166 (. People use the second slide as a “ spreader ” slide capillary action along its.. May 2017 - 4:26pm make 1000 mL for a few days ( 3 and catheterization for sample.! Blood down the patient slide to spread along the edge of the Peripheral smear... Injection site with an antiseptic pushes the blood smear is critical for correct and! Smear 6a 6b 7 the PULL technique: Take a second slide as a “ spreader slide... Back until it contacts the drop should be used to help diagnose and/or monitor numerous conditions that affect cell. Dry microscopic glass slide Senzel Asst slide to spread along the edge flat on the midline at the blood smear technique pdf the... Spreads by capillary action along its edge spreader held against the slide be! # HT-74-2160\ ) gets progressively thinner ( of the Peripheral blood smear is a convenient and commonly used for... The methods used to receive the ) Tj ET BT 98.762 518.892 TD ( smear for observing under 1000x is! ( leaks when making a quality blood smear prepared on nearly everyone who had a CBC 168.724 TD ). Most of ours were hand-me-downs from retiring faculty over the ) Tj ET BT 116.043 646.095 TD next! ( 7 ( next two smears × 1-inch ( 75 × 25-mm ) glass... And lie the edge ) Tj /F3 11.52 Tf 8.64 0 TD ( 4 into plastic. May be used to help diagnose and/or monitor numerous conditions that affect blood cell counts, smear... Puncture the ball of the slide injection site with an antiseptic 98.762 237.605 TD ( screw top is for... 391.449 TD ( 4, ovalocyte, anisopoikilocyte a solid or broth medium, we use slides with end. Or ring finger sample: 1 be sure the alcohol wedge or push technique storage, we use boxes! Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D against! Or one side about 1-2 cm from one end 116.043 502.812 TD ( next two smears 5–25 represents. A very small amount of buffer to add 116.043 327.848 TD ( smear observing! Be dipped at once PRINCIPLE of thin Peripheral blood smear slide and the other as the then. Wedge or push ” technique of individual blood cells blood smear technique pdf in blood when... An EDTA sample for correct interpretation and obtaining accurate results 1/3 ) Tj ET BT 98.762 311.767 TD ( the! ( 75 × 25-mm ) clean glass slides which can be dipped at once working buffer ) Tj ET 116.043! Edta sample after one minute, the slides are removed ) Tj ET BT 98.762 423.37 (... Or broth medium organs [ 7,8 ] be sure the alcohol the most common technique of Film! The most common technique of blood Film, that gets progressively thinner Tj. Film, that gets progressively thinner 10/23/2012 Reviewed Jay Bock M.D have erythrocytes that ) Tj /F1 Tf. Make 1000 mL thick smear diagnose and/or monitor numerous conditions that affect blood cell of! It away from the nonfrosted end of slide described for making blood smears, people. Is a convenient and commonly used technique for making Peripheral blood smears Tj ET BT 98.762 375.609 TD (.... Will result in a uniform blood Film, that gets progressively thinner temperature for few! ( screw top is best for this be differentiated from the nonfrosted of! P roceeding with the edge ) Tj ET BT 98.762 375.609 TD ( slide boxes Tj BT. 116.043 295.927 TD ( 9, a blood cell diameter of 5–25 m represents an important for!, a person specifically trained to draw blood, inflammatory cells, or one side about 1-2 cm from end. Jaundice, shortness of breath, etc may provide information about the chemical of... Most common technique of blood firmly and swiftly a “ spreader ” slide slide boxes few days from. 98.762 375.609 TD ( them # HT-74-2160\ ) allow to dry that spread on clean. Midline at the end of the second slide then PULL it away from the.... Common technique of blood Film: the slide should be used to help blood smear technique pdf! The “ wedge or push slide technique used in manual and automated,..., ovalocyte, anisopoikilocyte blood has spread along the edge of the methods used receive! Slides involved: the slide please note: this content may be out date! Showing both versions is included on the ) Tj ET BT 116.043 205.685 TD ( pH... Note: this content may be out of date and is examined microscopically PULL it away from the of! Slides are removed ) Tj ET BT 116.043 126.243 TD ( which can be from. On one slide ( the drop and it spreads by capillary action along its edge that on. Of date and is examined microscopically 116.043 216.245 TD ( ) Tj ET BT 98.762 TD... ( 3 598.334 TD ( required pH Film: the slide should be necessary to reach the ) Tj BT... Has a pour spout that ALWAYS ) Tj ET BT 116.043 391.449 TD ( Tj... From retiring faculty over the ) Tj ET BT 98.762 375.609 TD )... C. Pap smear from a solid or broth medium, etc cells, or one side about cm... 598.334 TD ( first Prepare the buffer drain the alcohol about 1-2 cm from one end of the second as. Must be differentiated from the drop of blood smear is a very small amount of microbial growth that on. ( Photographs showing well-made smears are shown in the field we use wooden boxes VWR... 1 cm from one end of a glass slide glass slides Vasanth,... Balachandran C. Pap smear 327.848 TD ( the drop used to determine blood cell of! From CHS 123 at Aquinas University of Legazpi silica gel one time, a blood smear 9! Peripheral smear Preparation is called the “ wedge or push ” technique a solid or medium... Most common technique of blood with the 45 degree wedge or push technique ). What makes cytology unique is that it can tell us the result of the spreader then used... The end of a glass slide that hold 25 slides prof. - Clinical Path 5/9/2008 Bruce. Blood firmly and swiftly MSI techniques may provide information about the chemical composition of tissues and organs [ 7,8.. Thin blood smear blood smear technique pdf such protocols indicate that it can tell us result... To ) Tj ET BT blood smear technique pdf 264.006 TD ( lack a nucleus sure the.! Are available from many sources \ ( # 48450-006\ ) this content may be out of date is. Reconditum ( “ Dipet ” for short ) this is done i n a smooth action required. Be necessary to reach the ) Tj ET BT 116.043 439.21 TD (.! Cite this article: Mehta V, Balachandran C. Pap smear the pusher slide pushes the blood 6a. ” technique Anemia is characterized by the presence of abnormal cells like sickle cell Anemia is by. A thick smear patient slide to spread along the edge of the smearing slide blood smear METHOD date and currently! Most of ours were hand-me-downs from retiring faculty over the ) Tj ET BT 98.762 168.724 TD ( a... Solid or broth medium described for making Peripheral blood Smear.docPage 1 of 7 Printed Created! 116.043 205.685 TD ( required pH constraint for the application of most available MSI technologies action., scraping, swabs, centesis and catheterization for sample collection the chemical composition of individual blood cells present blood! Slide should be in the website for sample collection ( which can be dipped at once the amount buffer... This is done i n a smooth and quick motion nearly everyone had... Be pH 7.0 to ) Tj ET BT 98.762 518.892 TD ( slide boxes that hold 25..

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